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KMID : 0545120020120020349
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 2 p.349 ~ p.353
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Effect of Trehalose on Bioluminescence and Viability of Freeze-Dried Bacterial Cells
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Park, Ji Eun
Lee, Kyu Ho/Jahng, Deok Jin
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Abstract
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Two recombinant bacteria containing luxAB showed an increased tolerance to stresses associated with lyophilization, when the cells were freeze-dried in the presence of trehalose. In the case of a recombinant, UV2, only 2.5% of the original bioluminescence and 2.7% of the cell viability were restored after 4 h of freeze-drying without trehalose, which implies that the cells were heavily damaged during the dehydration. To improve these losses, trehalose was added before freeze-drying using different modes. Trehalose increased the bioluminescence and the viability of freeze-dried UV2 under all conditions tested, and it was also observed that the addition of trehalose to the cultures (final concentration of 0.08 M) for 15 min before the freeze-drying resulted in the restoration of 45% of the original bioluminescence and 50% of the cell viability. Trehalose also showed a similar efficacy with the other luminescent recombinant, YH9. Therefore, it was tentatively concluded that trehalose played a role as a protective agent in the freeze-drying of bacterial cells.
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